ABSTRACT
Objective: To study the effects of extracts from Aconitum kongboense on gut immunity of Drosophila melanogaster. Methods: Flies fed with standard cornmeal-yeast medium were used as control, and the treatment groups contained 1% of A. kongboense aqueous extracts in standard medium. D. melanogaster was treated by some toxic compounds such as sodium dodecyl sulfate (SDS), DSS, H2O2, and Paraquat (PQ) with or without extracts of A. kongboense, and the effect of A. kongboense on the survival rate, intestinal epithelium cell death, relative contents of reactive oxygen species (ROS) in intestinal epithelium cells, and the number of intestinal epithelium cells and intestinal morphology changes were analyzed. Results: A. kongboense could significantly improve the survival rate of SDS, DSS, H2O2, and PQ-infected D. melanogaster, reduce the intestinal epithelial cell death and the level of intestinal ROS, decrease the over-proliferation of intestinal epithelial cell and the formation of melanotic masses. Conclusion: The extracts of A. kongboense could significantly improve the injury induced by chemical substances. This result provides experimental foundation for the exploitation of A. kongboense.
ABSTRACT
Objective: To identify Aconitum pendulum Busch. and Aconitum kongboense Lauener. Methods: Aconitum pendulum Busch. and Aconitum kongboense Lauener were identified by microscopical identification and TLC. And the contents of aconitine in the two herbs were determined by HPLC. Results:Microscopical identification showed they were different from each other, and aconitine was used as the reference component. Using the solution consisting of hexane ∶ethyl acetate ∶diethylamine (10 ∶6 ∶0. 8) as the developing solvent, the compositions in the two herbs were different in TLC. The content of aconitine in Aconitum pendulum Busch was 0. 71 mg· g-1 , while that in Aconitum kongboense Lauener was only 0. 03 mg·g-1 . Conclusion:Aconitum pendulum Busch. and Aconitum kong-boense Lauener Identified by microscopical identification, TLC and HPLC,SHOW NOTABLE differences between them, and Aconitum kongboense Lauener should not be used as Aconitum pendulum Busch.